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實驗室簡介： 分子酶學與合成生物學實驗室依托于上海交通大學微生物代謝國家重點實驗室，致力于解析酶催化與分子進化規(guī)律，發(fā)展酶定向進化技術體系，并開發(fā)酶在工業(yè)、醫(yī)藥等領域的重大創(chuàng)新應用。 實驗室承擔了國家973計劃項目、863項目、國家重大研究計劃項目、自然科學基金委重大儀器專項、自然科學基金等研究任務。在science adv., nature commun., angew. chem., jacs, anal chem, j. biol chem, 等著名雜志發(fā)表多篇論文。 代表性論文： 1)xi deng, liuqing chen, mohan hei, tiangang liu, yan feng, guang-yu yang. structure-guided reshaping of the acyl binding pocket of 'tesa thioesterase enhances octanoic acid production in e. coli, metab eng. 2020, 61:24-32. 2)yumeng tan, yong zhang, yunbin han, hao liu, haifeng chen, fuqiang ma, stephen g withers, yan feng, guangyu yang. directed evolution of an α1,3-fucosyltransferase using a single-cell ultrahigh-throughput screening method. science advances, 2019, eaaw8451 3)fuqiang ma#, meng ting chung#, yuan yao, robert nidet, lap man lee, allen p. liu, yan feng, katsuo kurabayashi*, guang-yu yang*. efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform. nature communications, 2018, 9:1030. (#equal contribution) 4)hoque ma, zhang y, chen l, yang g, khatun ma, chen h, hao l, feng y*. stepwise loop insertion strategy for active site remodeling to generate novel enzyme functions. acs chemical biology. 2017, 12(5):1188-1193. 5)zhuang y#, yang gy#, chen x, liu q, zhang x, deng z, feng y*. biosynthesis of plant-derived ginsenoside rh2 in yeast via repurposing a key promiscuous microbial enzyme. metabolic engineering. 2017, 42:25-32. (#equal contribution) 6)han yb, chen lq, li z, tan ym, feng y, yang gy*. structural insights into the broad substrate specificity of a novel endo-glycoceramidase i belonging to a new subfamily of gh5 glycosidase. j biol chem. 2017, 292(12):4789-4800. 研究課題 1. 利用結構生物學、家族進化樹分析、祖先基因重構等方法揭示自然界關鍵酶家族的進化規(guī)律，探討酶高效的催化功能與底物選擇性的形成機制與調(diào)控規(guī)律； 2. 開發(fā)蛋白質(zhì)從頭設計、微流控酶活性超高通量篩選等酶分子設計與定向進化的新技術方法，提升工程化酶人工構建與優(yōu)化的效率； 3. 針對具有重大實際應用需求對關鍵酶進行酶分子設計與定向進化，提高酶對非天然底物的選擇性、催化效率及其對環(huán)境的適應性，促進酶在生物醫(yī)藥領域的應用。